Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

SKU: orb2667856

Description

The Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit is designed to quantify Vascular Cell Adhesion Molecule 1 (VCAM1) in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.625-40 µg/mL and a sensitivity of 0.27 µg/mL.

Research Area

Cardiovascular Research; Immunology & Inflammation; Cell Biology

Images & Validation

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Application Notes

standard: 40 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VCAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VCAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.625-40 µg/mL
Sensitivity	0.27 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD106; INCAM-100; L1CAM

Quality Guarantee

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Quick Database Links

UniProt

P29534

UniProt Details

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No UniProt data available

Protocol Information

Find More Protocols

Reactivity:	Rat
Dynamic Range:	0.156-10ng/ml
Sensitivity:	0.094ng/ml

Reactivity:	Rat
Dynamic Range:	12.5-800pg/mL
Sensitivity:	5.13pg/mL

Reactivity:	Rat
Dynamic Range:	12.5-800 pg/mL
Sensitivity:	5.2 pg/mL

Reactivity:	Rat
Dynamic Range:	12.5-800ng/mL
Sensitivity:	5.25ng/mL

Rat Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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