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IL2RA Antibody (Biotin)
SKU: orb1671471
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-2 Receptor alpha. Anti-Human sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated. |
| Target | IL2RA |
| Purification | sIL-2 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−soluble IL-2 receptor, TAC-antigen, CD25 antigen
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To detect Human sIL-2 Receptor alpha by sandwich ELISA (using 100 μl/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sIL-2 Receptor alpha as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human sIL-2 Receptor alpha.
To detect Human sIL-2 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for Recombinant Human sIL-2 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL2RA Antibody (Biotin) (orb1671471)
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