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Recombinant HER2 Fab Antibody
SKU: orb420301
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 to 1:100,000, FC: 1:100, WB: 1:2,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Recombinant |
| Isotype | IgG Fab |
| Clone No. | Abz518 |
| Source/Expression System | E. coli |
| Purity | Humanized Recombinant HER2 Fab fragment was expressed in E. coli cells. The purity was estimated to be > 90% by SDS-PAGE analysis. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 2-8° C. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 0.59 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CD340, cluster of differentiation 340, ERBB2 (human), human epidermal growth factor receptor 2, HER2/neu, recombinant Monoclonal HER2, RabMAb HER2, Trastuzumab, Herceptin, Humanized Antibody, Recombinant Anti-HER2 Fab antibody, HER-2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, p185erbB2
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ELISA results show the sensitivity of Humanized Recombinant Anti-human HER2 Fab fragment Antibody tested against purified HER2 protein. Each well was coated in duplicate with 1.0 µg of HER2. The starting dilution of antibody was 5.0 µg/ml.
Flow Cytometry of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Primary Antibody: Recombinant Anti-HER2 Fab fragment (red line) recognizes HER2 on SK-OV-3 cells while using Anti-human kappa FITC secondary antibody at 0.2 µl with a 56.8% positive cells. Controls used: (black line) no primary antibody, no secondary antibody, 0.6%; (green line) no primary antibody, Anti-human kappa FITC secondary antibody at 0.2 µl, 1.0%.
SDS-PAGE of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1: Molecular Weight Ladder. Lane 2: reduced recombinant HER2 Fab fragment. Lane 3: non-reduced recombinant HER2 Fab fragment.
Western Blot of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1 and 8: Molecular Weight Marker. Lane 2 and 6: 0.02 µg HER2. Lane 3 and 7: 20 µg non-spiked SKOV-3 lysate. Lane 4 and 9: 20 µg SKOV-3 lysate spiked with 0.05 µg HER2. Lane 5 and 10: 20 µg SKOV-3 lysate spiked with 0.02 µg HER2. Primary Antibody: Anti-HER2 Fab incubated with lanes 1-5 only. Secondatry Antibody: Anti-F(ab)'2 HRP conjugate at 1:5000. Anti-HER2 Fab antibody observed in native HER2 and spiked HER2 in SKOV-3 cell lysates.
Western Blot of Humanized Recombinant Anti-human HER2 Fab fragment Antibody. Lane 1: Molecular Weight Ladder. Lane 2, 5, and 8: Empty. Lane 3, 6, and 9: HER2 reduced. Lane 4, 7, 10: HER2 non-reduced. Protein Load: Lane 3-4: 0.5 µg. Lane 6-7: 0.1 µg. Lane 9-10: 0.02 µg. Primary Antibody: anti-HER2 Fab at 1:2000 for 2 hours at 4°C. Secondary Antibody: Anti-human kappa FITC conjugate at 1:1000 for 2 hours at 4°C. Exposure time 60s.
Quick Database Links
UniProt
RefSeq (Protein):NP_001005862.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001005862.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Recombinant HER2 Fab Antibody (orb420301)
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