Recombinant Bcl2 Antibody

SKU: orb2640495

Description

Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bcl2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rBCL2/782
Immunogen	Recombinant full-length human protein was used as the immunogen for the recombinant Bcl2 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC testing of human follicular lymphoma with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Bcl2 antibody (clone rBCL2/782) as confirmation of integrity and purity.

IHC testing of human tonsil tissue with recombinant Bcl2 antibody (rBCL2/782). HIER: boil tissue sections in 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.

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UniProt

P10415

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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