Recombinant CAD Antibody / CALD1 / Caldesmon

SKU: orb2635711

Description

Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells.

Images & Validation

−

Item 1 of 3

Tested Applications	IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CAD antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CALD1/7024R
Immunogen	Recombinant human full-length Caldesmon protein was used as the immunogen for the recombinant CAD antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Recombinant CAD Antibody / CALD1 / Caldesmon

Western blot testing of human ovary tissue lysate using recombinant CAD antibody (clone CALD1/7024R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

IHC staining of FFPE human colon tissue with recombinant CAD antibody (clone CALD1/7024R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CAD antibody (clone CALD1/7024R) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q05682

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

Find More Protocols