Recombinant CD2 Antibody

SKU: orb2635607

Description

CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD2 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	LFA2/3417R
Immunogen	A recombinant protein fragment from the N-terminal region was used as the immunogen for the recombinant CD2 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.

IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P06729

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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