Recombinant CD59 Antibody

SKU: orb2635672

Description

CD59 regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction.

Images & Validation

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Item 1 of 5

Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD59 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	MACIF/7021R
Immunogen	Recombinant human full-length CD59 protein was used as the immunogen for the recombinant CD59 antibody.
Purification	Protein A affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human spleen tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2 ug/ml. Cytoplasmic and membranous staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD59 antibody (clone MACIF/7021R) as confirmation of integrity and purity.

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UniProt

P13987

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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