Recombinant COVID-19 S-trimer Protein (BF.7)

SKU: orb3068380

Description

Recombinant COVID-19 S-trimer Protein is produced by the mammalian expression system and the target gene encoding Cys15-Gln1208 is expressed with a 6His tag at the C-terminus. T19I, LPP24-26del, A27S, HV69-70del, G142D, V213G, G339D, R346T,S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K mutations were identified in the COVID-19 variant (known as BF.7 lineage) which emerged in Europe. Six proline substitutions and furin cleavage site substitutions are introduced to stabilize the trimeric prefusion state of COVID-19 S protein and abolish the furin cleavage site, respectively.

Images & Validation

−

Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Please minimize freeze-thaw cycles.

Key Properties

−

Source	Human cells
Reactivity	Virus
Purity	Greater than 95% as determined by reducing SDS-PAGE.

Storage & Handling

−

Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid in a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

−

SARS-CoV-2 S protein; SARS-CoV-2 Spike glycoprotein; SARS-CoV-2 S glycoprotein; SARS-CoV-2 S-trimer Protein

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

P0DTC2

NCBI Gene ID

43740568

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

View Protocol

Find More Protocols