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Recombinant Cynomolgus 4-1BBL Protein

SKU: orb2992607

Description

Recombinant Cynomolgus 4-1BB ligand is produced by our Mammalian expression system and the target gene encoding Arg68-Glu251 is expressed with a 6His, Flag tag at N-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginCynomolgus
Biological ActivityBLI: Loaded Recombinant Cynomolgus 4-1BBL (N-6His-Flag) (Cat#orb2992607) on HIS1K Biosensor, can bind Recombinant Cynomolgus 4-1BB (C- Fc) (Cat#orb2994767) with an affinity constant of 69.5 pM as determined in BLI assay. (Regularly tested)
TagN-6His-Flag
Molecular WeightPredicted: 24.4 KDa. Observed: 24-28 KDa, reducing conditions
Expression RegionArg68-Glu251
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/μg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9
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UniProt Details

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Recombinant Cynomolgus 4-1BBL Protein (orb2992607)

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