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KRT7 Antibody

SKU: orb1274234

Description

KRT7 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC-P
ReactivityHuman
Application Notes
Flow Cytometry: 0.5-1ug/10e6 cellsImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Cytokeratin 7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Clone No.KRT7/1499R
ImmunogenFull-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.
TargetKRT7
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL

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KRT7 Antibody

IHC testing of FFPE human oviduct with recombinant Cytokeratin 7 antibody (clone KRT7/1499R). Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

KRT7 Antibody

Western blot testing of human HeLa cell lysate with recombinant Cytokeratin 7 antibody (clone KRT7/1499R).

KRT7 Antibody

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 7 antibody (clone KRT7/1499R); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.

KRT7 Antibody

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 7 antibody (clone KRT7/1499R) as confirmation of integrity and purity.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

KRT7 Antibody (orb1274234)

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Available Sizes

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100 μg
$ 1,070.00
DispatchUsually dispatched within 5-10 working days
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