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GPC3 Antibody

SKU: orb1274232

Description

GPC3 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC-P
ReactivityHuman, Rat
Application Notes
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, kappa
Clone No.GPC3/1534R
ImmunogenA partial human protein was used as the immunogen for the recombinant Glypican-3 antibody.
TargetGPC3
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5

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GPC3 Antibody

IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody (clone GPC3/1534R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

GPC3 Antibody (orb1274232)

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Available Sizes

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100 μg
$ 1,070.00
DispatchUsually dispatched within 5-10 working days
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