GPC3/Glypican-3 Antibody

SKU: orb1806346

Description

Anti-GPC3 / Glypican-3 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Clonality	Monoclonal
Target	GPC3 / Glypican-3
Source/Expression System	CHO
Molecular Weight	145.5 kDa
Purity	>95%
Purification	Protein A
Endotoxin	<0.001 EU/ug
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100 mM Proline/Acetic acid 20mM Arginine pH 5.0
Concentration	Batch dependent
Disclaimer	For research use only

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Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab)is more than 95% ;determined by SEC-HPLC.

Immobilized human GPC3; His tag at 2 μg/mL can bind Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab);EC50=0.008741μg/mL.

Human GPC3 CHOS cells were stained with Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC210=2.142 nM.

The endocytosis ratio codrituzumab by HuH-7 increased with the increase of antibody concentration; and the Internalization Rate (%) reached 40%at antibody concentration of 1 nM.

Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab)-induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.

Quick Database Links

Gene Symbol

GPC3 / Glypican-3

UniProt

P51654

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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