GPC3/Glypican 3 Antibody

SKU: orb1535790

Description

Images & Validation

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Tested Applications	IHC, IHC-P, WB
Dilution range	IHC (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000)
Reactivity	Human, Mouse
Application Notes	Further information: The predicted MW is 59kDa/65kDa/68kDa, while the observed MW by Western blot was 70kDa.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human GPC3 (NP_004475.1). VEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEIS
Target	GPC3 / Glypican 3
Purification	Affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration	1.28 mg/ml
Disclaimer	For research use only

Alternative Names

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GPC3, DGSX, Glypican proteoglycan 3, Glypican 3, Glypican-3, GTR2-2, Intestinal protein OCI-5, SGB, SGBS1, SGBS, MXR7, OCI-5, OCI5, SDYS, Secreted glypican-3, Heparan sulphate proteoglycan

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of extracts of various cell lines, using GPC3 antibody.

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Gene Symbol

GPC3 / Glypican 3

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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