Recombinant HSP27 Antibody / HSPB1

SKU: orb2635136

Description

HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of therecombinant HSP27 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	HSPB1/7038R
Immunogen	Recombinant human full-length protein was used as the immunogen for the recombinant HSP27 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human prostate tissue with recombinant HSP27 antibody (clone HSPB1/7038R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/7038R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04792

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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