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Recombinant Human 4-1BB Protein
SKU: orb2994490
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human 4-1BB-Fc-His(Cat#orb2994490) at 2μg/ml (100 μl/well)can bind Anti-Human CD137 mAb(Cat#orb2993114).The ED50 of Anti-Human CD137 mAb(Cat#orb2993114) is 0.41ug/ml.. BLI: Loaded Human 4-1BB-Fc-His(Cat#orb2994490) on HIS1K Biosensor, can bind Anti-Human CD137 mAb(Cat#orb2993114) with an affinity constant of 12.2 nM as determined in BLI assay. |
| Tag | C-Fc-6His |
| Molecular Weight | Predicted: 44 KDa. Observed: 55-75 KDa, reducing conditions |
| Expression Region | Leu24-Gln186 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA
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Recombinant Human 4-1BB Protein (orb2994490)
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