Recombinant Human ApoE3 Protein

SKU: orb2994564

Description

Recombinant Human Apolipoprotein E3 is produced by our Mammalian expression system and the target gene encoding Lys19-His317 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Recombinant Human ApoE3 (C-6His)(Cat# orb2994564) at 2μg/ml (100 μl/well) can bind Recombinant Human TREM2 (C-Fc)(Cat# orb2993662). The EC50 of Recombinant Human TREM2 (C-Fc)(Cat# orb2993662) is 41.52ng/ml.(Regularly tested)
Tag	C-6His
Molecular Weight	Predicted: 35.3 KDa. Observed: 39 KDa, reducing conditions
Expression Region	Lys19-His317
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM Tris-HCl, 5% Trehalose,5% Mannitol, 0.02% Tween80, pH 8.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Apolipoprotein E;APOE;Apo-E;ApoE3

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UniProt

P02649

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