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Human CD16b
SKU: orb2994750
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human IgG1 Fc (Cat#orb2993960) on Protein-A Biosensor, can bind Human CD16b (Cat#orb2994750) with an affinity constant of 5.34 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 22.7 KDa. Observed: 35-65 KDa, reducing conditions |
| Expression Region | Gly17-Gln208 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; FcR-10; IgG Fc receptor III-1; FCG3; FCGR3; CD16b; FCGR3B
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 48.8 KDa. Observed: 70 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μg

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Human CD16b (orb2994750)
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