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Recombinant Human CD19 Protein
SKU: orb2994989
Description
Images & Validation
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Key Properties
−| Expression System | CHO Stable Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human FMC63 at 2μg/ml (100 μl/well) can bind Human CD19-Fc (Cat#orb2994989). The ED50 of Recombinant Human CD19-Fc (Cat#orb2994989) is 55.28 ng/ml.. BLI: Loaded Anti-Human CD19 mAb-Fc(Cat#orb2993102) on AMC Biosensor, can bind Human CD19-FC(Cat#orb2994989) with an affinity constant of 2.72 nM as determined in BLI assay. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 57.3 KDa. Observed: 80-95 KDa, reducing conditions |
| Expression Region | Pro20-Lys291 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19
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Recombinant Human CD19 Protein (orb2994989)
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