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Human CD3D&CD3E Heterodimer
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Anti-Human CD3E mAb-mFc(Cat#orb2992718) on AMC Biosensor, can bind Human CD3D&CD3E Heterodimer C-Fc-Flag&C-Fc-6His(Cat#orb2993825) with an affinity constant of -3 nM as determined in BLI assay. |
| Tag | C-Fc-Flag&C-Fc-6His |
| Molecular Weight | Predicted: 37&39.2 KDa. Observed: 40-60 KDa, reducing conditions |
| Expression Region | Phe22-Ala105&Asp23-Asp126 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Human CD3D&CD3E Heterodimer (C-6His) [orb2993864]
Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 10.6&12.8 KDa. Observed: 16-30 KDa, reducing conditions
10 μg, 50 μg, 1 mg, 500 μgCynomolgus CD3D&CD3E Heterodimer [orb2993714]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37&38.3 KDa. Observed: 40-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman CD3D&CD3E Heterodimer V2 [orb2992888]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 14.6&16.0 KDa. Observed: 20&(22-38) KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Human CD3D&CD3E Heterodimer Protein, C-His [orb1141378]
≥95% as determined by SDS-PAGE
Human
50 μg, 10 μgRecombinant Human CD3D&CD3E Heterodimer Protein, C-Fc-Flag&C-Fc-His [orb1141377]
≥95% as determined by SDS-PAGE
Human
50 μg, 10 μg

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Human CD3D&CD3E Heterodimer (orb2993825)
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