Recombinant Human CD40L Protein

SKU: orb2994532

Description

Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Anti-Human CD40L mAb(Cat. No.:orb2993151) at 5 μg/mL (100 μL/well) can bind Human CD40L (Cat. No.:orb2994532). The EC50 of Human CD40L (Cat. No.:orb2994532) is ≤10 ng/mL.（QC Verified）. BLI: Loaded Human CD40-mFc(Cat#orb2994039) on AMC Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 10.2 nM as determined in BLI assay. Loaded Human CD40-Fc(Cat#orb2994010) on Protein A Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 3.29 nM as determined in BLI assay. Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L(Cat#orb2994532) with an affinity constant of 0.02 nM as determined in BLI assay.
Tag	Tag free
Molecular Weight	Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
Expression Region	Met113-Leu261
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Endotoxins	< 0.01 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

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UniProt

P29965

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No UniProt data available

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