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Recombinant Human CD47 Protein
SKU: orb2995144
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Anti-Human CD47 mAb-Fc(Cat#orb2993150) on Protein A Biosensor, can bind Human CD47-His(Cat#orb2995144) with an affinity constant of 0.22 nM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 14.76 KDa. Observed: 30-45 KDa, reducing conditions |
| Expression Region | Gln19-Pro139 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6
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Greater than 95% as determined by reducing SDS-PAGE.
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Recombinant Human CD47 Protein (orb2995144)
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