Recombinant Human Ciliary Neurotrophic Factor

SKU: orb1906246

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Description

Recombinant Human Ciliary Neurotrophic Factor

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Key Properties

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Source	Escherichia coli
Expression System	Escherichia coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 �� 103 IU/mg.
Molecular Weight	Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
Protein Sequence	MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1 EU/ug of rHuCNTF as determined by LAL method.

Storage & Handling

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Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Form/Appearance	Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Disclaimer	For research use only

Alternative Names

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CNTF

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UniProt

P26441

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