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Human EGFR
SKU: orb2994530
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Anti-Human EGFR mAb-Fc (Cat#orb2993008) on Pro A Biosensor, can bind Recombinant Human EGFR-His (Cat#orb2994530) with an affinity constant of 7.23 nM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 69.6 KDa. Observed: 90-120 KDa, reducing conditions |
| Expression Region | Leu25-Ser645 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 50mM Nacl, 0.05% Tween80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR;ERBB; ERBB1; HER1
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Human EGFR (orb2994530)
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