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Recombinant human ErbB2 / Her2 protein, C-His-Avi (HEK293)
SKU: orb1516438
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Description
Research Area
Cancer Research, Cell Biology, Epidermal Growth Factor, Receptor Tyrosne Kinases, Tumor Biology
Images & Validation
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Key Properties
−| Expression System | HEK293 cell |
|---|---|
| Target | Receptor tyrosine-protein kinase erbB-2 |
| Tag | C-His-Avi |
| Molecular Weight | Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
| Protein Sequence | 23-652/1255 |
| Purification | AC |
| Purity | >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC |
| Endotoxins | <1.0 EU/μg as determined by LAL |
Storage & Handling
−| Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| Concentration | >0.5 mg/ml |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Disclaimer | For research use only |
Alternative Names
−erbB-2 isoform 2; HER2 receptor; Erbb2 protein; CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; Erb B2; erbb2; HER 2; Her-2; HER 2/neu; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1; Tyrosine kinase type cell surface receptor HER2; v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian); ERBB2_HUMAN; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; HER2; Recombinant human ErbB2 / Her2 protein, C-His-Avi (HEK293)
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Immobilized Human Her2, His Tag at 0.2 µg/ml (100 µl/Well). Dose response curve for Anti-Her2 Ab. Trastuzumab, with the EC50 of 17.4 ng/ml determined by ELISA.
Immobilized Human Her2, His Tag at 0.2 µg/ml (100 µl/Well). Dose response curve for Anti-Her2 Ab. with the EC50 of 15.9 ng/ml/15.0 ng/ml/15.7 ng/ml determined by ELISA.
Recombinant Human ErbB2/Her2 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Recombinant Human ErbB2/Her2 Protein is greater than 95% as determined by SEC-HPLC.
Quick Database Links
Gene Symbol
Receptor tyrosine-protein kinase erbB-2
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Protocol Information
Recombinant human ErbB2 / Her2 protein, C-His-Avi (HEK293) (orb1516438)
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