Recombinant human ErbB3 / Her3 protein, C-His-Avi (HEK293)

SKU: orb1516436

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Description

Recombinant human ErbB3 / Her3 protein, C-His-Avi (HEK293), with a concentration of >0.5 mg/ml, expressed in HEK293 cell, and a purity of >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC. It was purified by AC, and has a C-His-Avi tag. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.

Research Area

Cancer Research, Cell Biology, Epidermal Growth Factor, G Protein Signaling Pathway, Receptor Tyrosne Kinases, Signal Transduction

Images & Validation

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Key Properties

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Expression System	HEK293 cell
Target	Receptor tyrosine-protein kinase erbB-3
Tag	C-His-Avi
Molecular Weight	Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Protein Sequence	20-643/1342
Purification	AC
Purity	>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Endotoxins	<1.0 EU/μg as determined by LAL

Storage & Handling

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Storage	Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
Concentration	>0.5 mg/ml
Reconstitution	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Disclaimer	For research use only

Alternative Names

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erbB-2 isoform 2; HER2 receptor; Erbb2 protein; CerbB2; c erb B2; C erbB 2; C-erbB2; CD340; Erb B2; erbb2; HER 2; Her-2; HER 2/neu; HER2; Her2/neu; Herstatin; MLN 19; MLN19; NEU; NEU Proto Oncogene; Neuro Glioblastoma Derived Oncogene Homolog; NGL; p185 ErbB2; p185erbB2; Receptor Protein Tyrosine Kinase ErbB2 Precursor; Receptor tyrosine protein kinase erbB 2; TKR1; Tyrosine kinase type cell surface receptor HER2; v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian); ERBB2_HUMAN; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Recombinant human ErbB3 / Her3 protein, C-His-Avi (HEK293)

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Recombinant human ErbB3 / Her3 protein, C-His-Avi (HEK293)

Immobilized Human Her3 at 1 ug/ml (100 ul/Well). Dose response curve for Anti-Her3 Ab. with the EC50 of 0.2 ug/ml determined by ELISA.

Recombinant Her3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Her3 is greater than 95% as determined by SEC-HPLC.

Quick Database Links

Gene Symbol

Receptor tyrosine-protein kinase erbB-3

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