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Recombinant Human FSTL1 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Tag | C-6His |
| Molecular Weight | Predicted: 33.5 KDa. Observed: 48 KDa, reducing conditions |
| Expression Region | Glu21-Ile308 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 33 KDa. Observed: 39 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human FSTL1 Protein [orb2994643]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.8 KDa. Observed: 30-38 KDa, reducing conditions
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 59.6 KDa. Observed: 70-85 KDa, reducing conditions
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Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of FSTL1-His is approximately 35-55 kDa due to glycosylation.
E.coli
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UniProt Details
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Protocol Information
Recombinant Human FSTL1 Protein (orb2994687)
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