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Description
Research Area
Neuroscience
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Tag | N-6His |
| Expression Region | Leu292-Met432 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 18.7 KDa. Observed: 17 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 0.05% Tween 80, pH 8.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Glial Fibrillary Acidic Protein; GFAP
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 37.2 kDa after removal of the signal peptide. The apparent molecular mass of His-GFAP(68-377) is approximately 35-55 kDa due to glycosylation.
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Quick Database Links
UniProt
UniProt Details
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Datasheet
Product Information
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Recombinant Human GFAP Protein (orb2995191)
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