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Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human GPNMB (C-6His)(Cat#orb2995035) at 2μg/ml (100 μl/well) can bind Anti-Human GPNMB Antibody (GLE_bio, Research Grade)(Cat#orb2992498).The ED50 of Anti-Human GPNMB Antibody (GLE_bio, Research Grade)(Cat#orb2992498) can bind is 10.84 ng/ml. (Regularly tested). |
| Tag | C-6His |
| Expression Region | Lys23-Pro486 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 53.09 KDa. Observed: 80-120 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−GPNMB Mouse Monoclonal Antibody [orb2988355]
IHC, WB
Human
Mouse
Monoclonal
Unconjugated
200 μl, 100 μl, 50 μl, 30 μlHuman GPNMB Protein, His Tag [orb1743304]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 53.9 kDa after removal of the signal peptide. The apparent molecular mass of GPNMB-His is approximately 70-130 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgRecombinant human GPNMB protein, C-His (HEK293) [orb1808076]
>90% as determined by SDS-PAGE
19.4 kDa
100 μg, 500 μg, 20 μgRecombinant Human GPNMB Protein, C-His [orb2966377]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
55.43 kDa
1 mg, 50 μg, 100 μgRecombinant Human GPNMB Protein, C-His [orb3153240]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
53.60 kDa
50 μg, 100 μg, 1 mg

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