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Recombinant human IFN gamma Antibody

SKU: orb2657853

Description

Recombinant human IFN gamma Antibody

Images & Validation

Tested ApplicationsELISA, Functional Studies
Application Notes
Recombinant protein IFN gamma is expressed in S2 cell (Drosophila). DNA sequence encoding 143 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18 kDa.ELISA: Recombinant protein used as a standard in ELISA may not fully reflect the properties of the native protein, which can lead to inaccurate quantification of its concentration in samples.

Key Properties

Antibody TypeRecombinant Antibody
ClonalityRecombinant
PurificationPurified by affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Recombinant human IFN gamma Antibody

Recombinant Human IFN gamma induces cytotoxicity in HT-29 human colon adenocarcinoma cell line. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 71.5 ng/ml.

Recombinant human IFN gamma Antibody

Recombinant Human IFN gamma induces cytotoxicity in HT-29 human colon adenocarcinoma cell line. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 71.5 ng/ml.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Recombinant human IFN gamma Antibody (orb2657853)

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0.1 mg
$ 300.00
DispatchUsually dispatched within 5-10 working days
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