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Description
Research Area
Immunology & Inflammation
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human IL-17A(Cat#orb2995247) at 2μg/ml (100 μl/well) can bind Anti-Human IL-17A mAb(Cat#orb2993149).The ED50 of Anti-Human IL-17A mAb(Cat#orb2993149) is 0.185 ug/ml.. BLI: Loaded Human IL-17RA-Fc(Cat#orb2994535) on Protein A Biosensor, can bind Human IL-17A(Cat#orb2995247) with an affinity constant of -3 nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686) on HIS1K Biosensor, can bind Human IL-17A(Cat#orb2995247) with an affinity constant of 0.50 nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc(Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17A(Cat#orb2995247) with an affinity constant of 0.79 nM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-7 ng/ml. |
| Tag | Tag free |
| Expression Region | Gly24-Ala155 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 15.26 KDa. Observed: 16 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A; CTLA8; IL17
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- Item 1 of 1
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 41.3 kDa after removal of the signal peptide.The apparent molecular mass of IL17A-hFc is approximately 35-55 kDa due to glycosylation.
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Recombinant Human IL-17A Protein (orb2995247)
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