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Human IL-17F
SKU: orb2995245
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human IL-17F(Cat#orb2995245) at 2μg/ml (100 μl/well)can bind Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686).The ED50 of Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686) is 0.11 ug/ml.. BLI: Loaded Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686) on HIS1K Biosensor, can bind Human IL-17F(Cat#orb2995245) with an affinity constant of 0.56 uM nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc(Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17F(Cat#orb2995245) with an affinity constant of 0.15uM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-40 ng/ml. |
| Tag | Tag free |
| Molecular Weight | Predicted: 15 KDa. Observed: 15 KDa, reducing conditions |
| Expression Region | Arg31-Gln163 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-17F; IL-17F; Cytokine ML-1; IL17F
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100 μg Human IL-17F [orb2994206]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.9 KDa. Observed: 18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-17A&17F [orb2994531]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC.(QC verified)
Predicted: 15.1&16 KDa. Observed: 15-25 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgBiotinylated Human IL-17A&17F [orb2993677]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15.1&17.8 KDa. Observed: 15-22 KDa, reducing conditions
20 μg, 100 μg
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Human IL-17F (orb2995245)
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