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Human IL-2RB
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | BLI: Loaded Human IL-2RB-His(Cat#orb2994462) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002496) with an affinity constant of 0.27 uM as determined in BLI assay. (Regularly tested) Loaded Human IL-2RB-His(Cat#orb2994462) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 0.19 uM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 25.6 KDa. Observed: 35-40 KDa, reducing conditions |
| Expression Region | Ala27-Asp239 |
| Purity | SEC-HPLC: Greater than 90% as determined by SEC-HPLC.(QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Biotinylated Human IL-2RB [orb2993868]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.4 KDa. Observed: 35-45 KDa, reducing conditions
20 μg, 100 μgMouse IL-2RB [orb2993766]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 52.2 KDa. Observed: 70-110 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-2RB [orb2994461]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 51.7 KDa. Observed: 60-80 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-2 R beta&IL-2 R gamma Heterodimer [orb2992889]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 54&51 KDa. Observed: (57-72)&(85-115) KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μg

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Human IL-2RB (orb2994462)
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