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Human IL-4 RA
SKU: orb2994196
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human IL-4 RA-Fc(Cat#orb2994196) at 5μg/ml (100 μl/well)can bind Human IL-4 *(Cat#orb3002477) .*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-4 (Cat#orb3002477)is 2.43 ng/ml.. BLI: Loaded Human IL-4-His(Cat#orb3002435) on HIS1K Biosensor, can bind Human IL-4RA-Fc(Cat#orb2994196) with an affinity constant of 0.12 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells.The ED50 for this effect is 5-20 ng/ml. (QC verified) |
| Tag | C-Fc |
| Molecular Weight | Predicted: 50.2 KDa. Observed: 60-70 KDa, reducing conditions |
| Expression Region | Met26-Gln231 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA
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Predicted: 24.4 KDa. Observed: 35-60 KDa, reducing conditions
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
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Human IL-4 RA (orb2994196)
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