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Human IL-6RA
SKU: orb2993733
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Human IL6R Antibody (SAR_bio, Research Grade)(Cat#NC373) at 1μg/ml (100 μl/well) can bind Recombinant Human IL -6RA (C-Fc)(Cat#orb2993733)*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human IL -6RA (C-Fc)(Cat#orb2993733) is 1.78 ng/ml.(Regularly tested). BLI: Loaded Recombinant Rhesus Macaque IL-6 (C-6His) (Cat#orb2992743) on HIS1K Biosensor, can bind Recombinant Human IL-6RA (C-Fc) (Cat#orb2993733) with an affinity constant of 21.6 nM as determined in BLI assay. (Regularly tested) |
| Tag | C-Fc |
| Molecular Weight | Predicted: 65.4 KDa. Observed: 85-100 KDa, reducing conditions |
| Expression Region | Leu20-Pro365 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6R 1; Membrane glycoprotein 80; gp80; CD126
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Human IL-6RA (orb2993733)
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