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Recombinant Human Insulin-like Growth Factor-Binding Protein 3
SKU: orb1906279
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x103 IU/mg in the presence of 15 ng/ml of rHuIGF-II. |
| Molecular Weight | Approximately 28.8 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids. |
| Protein Sequence | GASSAGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG SVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rHuIGF-BP3 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−Growth-hormone-dependant Binding Protein, IBP-3, IGF-binding protein 3
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Recombinant Human Insulin-like Growth Factor-Binding Protein 3 (orb1906279)
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