Recombinant Human Interleukin-22 (rHu IL-22)

SKU: orb1495039

Description

IL-22 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

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Source	Escherichia coli.
Biological Activity	Determined by its ability to activate STAT following receptor ligand interaction.
Molecular Weight	Approximately 33.6 kDa,non-disulfide-linked homodimeric protein containing of two 146 amino acid polypeptide chains.
Protein Sequence	MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI
Purification	>97% by SDS-PAGE and HPLC analyses.
Purity	>97% by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1EU/mg of rHuIL-22 as determined by LAL method.

Storage & Handling

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Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 5.0.
Buffer/Preservatives	Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 5.0.
Disclaimer	For research use only

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