Recombinant Human Interleukin-4 (IL4) (Active)

SKU: orb2659090

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Description

This Recombinant Human Interleukin-4 (IL4) spans the amino acid sequence from region 25-153aa. Purity: Greater than 95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Homo sapiens (Human)
Biological Activity	①Measured by its binding ability in a functional ELISA. Immobilized Human IL4 at 2 μg/mL can bind Biotinylated Human IL4R (CSB-MP011661HU2-B). The EC50 is 7.926-9.836 ng/mL. ②Human IL4 captured on Protein A Chip can bind Recombinant Human IL4R(CSB-MP011661HU2-B) with an affinity constant of 17.1 nM as detected by MetaSPR Assay (WeSPRTM 200).
Tag	C-terminal hFc1-MyC-tagged
Molecular Weight	43.9 kDa
Expression Region	25-153aa
Protein Length	Full Length of Mature Protein
Protein Sequence	HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
Purity	Greater than 95% as determined by SDS-PAGE.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

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Recombinant Human Interleukin-4 (IL4) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Quick Database Links

UniProt

P05112

UniProt Details

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No UniProt data available

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