Recombinant Human Macrophage Colony Stimulating Factor (rHuM-CSF)

SKU: orb1495033

Description

M-CSF, also known as CSF-1, is a four α helical bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

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Source	Baculovirus infected Silkworm
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine M-NSF-60 cells is less than 3.0 ng/ml, corresponding to a Specific Activity of ＞3.3 x 105 IU/mg.
Molecular Weight	Approximately 42.0 kDa, a covalently linked homodimer, of two 21.0 kDa polypeptide monomers (1-149 amino acid of human M-CSF).
Protein Sequence	EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDKACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ
Purification	>95% by SDS-PAGE and HPLC analyses.
Purity	>95% by SDS-PAGE and HPLC analyses.
Endotoxins	Less than 1EU/mg of rHuM-CSF as determined by LAL method.

Storage & Handling

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Storage	This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Form/Appearance	Lyophilized from a 0.2mm filtered solution in 20mM PB, containing 1% HSA and 3% Mannitol.
Buffer/Preservatives	Lyophilized from a 0.2mm filtered solution in 20mM PB, containing 1% HSA and 3% Mannitol.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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