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Recombinant Human NCR1 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Anti-Human NKp46 mAb at 2 μg/ml (100 μl/well) can bind Recombinant Human NCR1 (C-6His) (Cat#orb2992787). The ED50 of Recombinant Human NCR1 (C-6His)(Cat#orb2992787) is 22.52 ng/ml. (Regularly tested).. BLI: Loaded Recombinant Anti-NKp46 mAb on HIS1K Biosensor, can bind Recombinant Human NCR1 (C-6His) (Cat#orb2992787) with an affinity constant of 1.58 pM as determined in BLI assay. (Regularly tested) |
| Tag | C-6His |
| Molecular Weight | Predicted: 27.2 KDa. Observed: 31-40 KDa, reducing conditions |
| Expression Region | Gln22-Asn254 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 53.5 KDa. Observed: 76 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgRecombinant Human NCR1 Protein [orb2994921]
Unconjugated
SDS-PAGE: Greater than 85% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 53.5 KDa. Observed: 55-80 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman NCR1 Protein, hFc Tag [orb1173746]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.5 kDa after removal of the signal peptide. The apparent molecular mass of NCR1-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant Human CD335/NCR1/NKp46 Protein, C-His [orb2961403]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
29.97 kDa
1 mg, 50 μg, 100 μg

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Recombinant Human NCR1 Protein (orb2992787)
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