Recombinant Human OX40L Protein

SKU: orb2994050

Description

Recombinant Human OX40 Ligand is produced by our Mammalian expression system and the target gene encoding Gln51-Leu183 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human OX40-Fc(Cat#orb2994422) at 8μg/ml (100 μl/well)can bind Human OX40L-mFc(Cat#orb2994050).The ED50 of Human OX40L-mFc(Cat#orb2994050)is 18.54 ng/ml.. BLI: Loaded Cynomolgus OX40-His(Cat#orb2994769) on HIS1K Biosensor, can bind Human OX40L-mFc(Cat#orb2994050) with an affinity constant of 0.05nM as determined in BLI assay.
Tag	C-mFc
Molecular Weight	Predicted: 42.2 KDa. Observed: 50-70 KDa, reducing conditions
Expression Region	Gln51-Leu183
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFSF4; CD252; TXGP1

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Human OX40 Protein, hFc-His tag [orb689383]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Human OX40L Protein [orb2992806]
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
MW:
Predicted: 44.2 KDa. Observed: 53-68 KDa, reducing conditions
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg
Recombinant Mouse OX40L Protein [orb2992812]
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
MW:
Predicted: 45.7 KDa. Observed: 50-68 KDa, reducing conditions
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg
Recombinant Human OX40L Protein [orb2994486]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 16.3 KDa. Observed: 20-30 KDa, reducing conditions
Sizes Available: 50 μg, 500 μg, 1 mg, 10 μg
Recombinant Human OX40 Protein [orb2994420]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 21 KDa. Observed: 38-45 KDa, reducing conditions
Sizes Available: 50 μg, 500 μg, 1 mg, 10 μg

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P23510

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