Recombinant Human OX40L Protein

SKU: orb2994486

Description

Recombinant Human OX40 Ligand is produced by our Mammalian expression system and the target gene encoding Gln51-Leu183 is expressed with a 6His tag at the N-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	BLI: Loaded Biotinylated Human OX40L-His(Cat#orb2994486) on AR2G Biosensor, can bind Human OX40-Fc(Cat#orb2994422) with an affinity constant of 70.3 nM as determined in BLI assay. (Regularly tested)
Tag	N-6His
Molecular Weight	Predicted: 16.3 KDa. Observed: 20-30 KDa, reducing conditions
Expression Region	Gln51-Leu183
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFSF4; CD252; TXGP1

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Human OX40 Protein, hFc-His tag [orb689383]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Human OX40L Protein [orb2992806]
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
MW:
Predicted: 44.2 KDa. Observed: 53-68 KDa, reducing conditions
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg
Recombinant Mouse OX40L Protein [orb2992812]
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
MW:
Predicted: 45.7 KDa. Observed: 50-68 KDa, reducing conditions
Sizes Available: 1 mg, 500 μg, 50 μg, 10 μg
Recombinant Human OX40 Protein [orb2994420]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 21 KDa. Observed: 38-45 KDa, reducing conditions
Sizes Available: 50 μg, 500 μg, 1 mg, 10 μg
Recombinant Human OX40 Protein [orb2994421]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 46.3 KDa. Observed: 40-60 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

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UniProt

P23510

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