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Recombinant Human Siglec-5 Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Tag | C-6His-Flag-Fc |
| Molecular Weight | Predicted: 74.1 KDa. Observed: 90-110 KDa, reducing conditions |
| Expression Region | Glu17-Thr434 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Histidine, 10% Trehalose, 2% Mannitol, 50mM NaCl, 0.05% Tween80, pH 5.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human Siglec-5 Protein [orb2993021]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 73.3 KDa. Observed: 90-110 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgHuman SIGLEC5 Protein, His Tag [orb1743336]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.2 kDa after removal of the signal peptide. The apparent molecular mass of SIGLEC5-His is approximately 55-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μgRecombinant Human CD170/SIGLEC5 Protein, C-His [orb2966474]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
49.07 kDa
1 mg, 50 μg, 100 μgRecombinant Human CD170/SIGLEC5 Protein, N-His [orb2969954]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
12.76 kDa
1 mg, 100 μg, 50 μgRecombinant Human CD170/SIGLEC5 Protein, C-Fc [orb2964853]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
74.50 kDa
1 mg, 50 μg, 100 μg

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Protocol Information
Recombinant Human Siglec-5 Protein (orb2995148)
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