Recombinant Human ST2 Protein

SKU: orb2994520

Description

Recombinant Human Interleukin-1 Receptor-like 1 is produced by our Mammalian expression system and the target gene encoding Lys19-Phe328 is expressed with a 6His tag at the C-terminus.

Images & Validation

−

Key Properties

−

Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human ST2-His(Cat#orb2994520) at 5μg/ml (100 μl/well) can bind Human IL-33-His(Cat#C233).The ED50 of Human IL-33-His(Cat#C233) is 8.44 ng/ml.. BLI: Loaded Human IL-33(Cat#orb2995234) on AR2G Biosensor, can bind Human ST2-His(Cat#orb2994520) with an affinity constant of 0.97nM as determined in BLI assay. Loaded Human sST2 Antibody-mFc(Cat#DA014) on AMC Biosensor, can bind Human ST2-His(Cat#orb2994520) with an affinity constant of -3 nM as determined in BLI assay.
Tag	C-6His
Molecular Weight	Predicted: 36 KDa. Observed: 50-85 KDa, reducing conditions
Expression Region	Lys19-Phe328
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Interleukin-1 receptor-like 1;Protein ST2;DER4; ST2; T1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

Q01638

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols