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Recombinant Human TfR Protein
SKU: orb2992644
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Anti-Huamn TFRC mAb (Trontinemab) at 2 μg/ml (100 μl/well) can bind Recombinant Human TfR (C-Fc) (Cat#orb2992644)*. *:Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human TfR (C-Fc) (Cat#orb2992644) is 3.001 ng/ml. (Regularly tested).. BLI: Loaded Human TFRC Antibody (Trontinemab, Research Grade) (Cat#orb2992592) on Pro-A Biosensor, can bind Recombinant Human TfR (N-Fc) (Cat#orb2992644) with an affinity constant of 20.4 nM as determined in BLI assay. (Regularly tested) |
| Tag | N-Fc |
| Molecular Weight | Predicted: 101 KDa. Observed: 95-120 KDa, reducing conditions |
| Expression Region | Cys89-Phe760 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | ＜ 1 EU/μg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90
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Recombinant Human TfR Protein (orb2992644)
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