Recombinant Human TNF RI Protein

SKU: orb2994315

Description

Recombinant Human Tumor Necrosis Factor Receptor I is produced by our Mammalian expression system and the target gene encoding Leu30-Thr211 is expressed with a human IgG1 Fc tag at the C-terminus.

Images & Validation

−

Key Properties

−

Expression System	Human Cells
Biological Origin	Human
Biological Activity	BLI: Loaded Recombinant Human TNF RI (C-Fc) (Cat#orb2994315) on Pro-A Biosensor, can bind Recombinant Human TNF alpha (Cat#orb3002485) with an affinity constant of 1.252 pM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce NF-κB reporter activity in HEK293 human embryonic kidney cells. Recombinant Human TNF RI (Cat# orb2994315) inhibits a constant dose of 0.5ng/mL of Recombinant TNF alpha(Cat#orb3002485). The IC50 for this effect is 2.7 ng/mL. (Regularly tested)
Tag	C-Fc
Molecular Weight	Predicted: 47.2 KDa. Observed: 60 KDa, reducing conditions
Expression Region	Leu30-Thr211
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Tumor necrosis factor receptor superfamily member 1A;TNFRSF1A;Tumor necrosis factor receptor 1; TNF-R1; TNF-RI; p55; p60; CD120a; TNFAR; TNFR1

Human TNFRSF1A Protein, hFc Tag [orb1291011]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 46.6 kDa after removal of the signal peptide. The apparent molecular mass of TNFRSF1A-hFc is approximately 55-70 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 50 μg, 100 μg, 10 μg

Recombinant Human CD120a/TNFRSF1A Protein, C-His [orb2969001]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>95% as determined by SDS-PAGE.
MW:
24.40 kDa
Sizes Available: 1 mg, 50 μg, 100 μg
Recombinant Human CD120a/TNFRSF1A Protein, N-His [orb2969002]
Applications:
ELISA, SDS-PAGE, WB
Purity:
>90% as determined by SDS-PAGE.
MW:
26.79 kDa
Sizes Available: 1 mg, 50 μg, 100 μg
Atrosimab Human Monoclonal Antibody [orb2976258]
Applications:
ELISA, FA, FACS, In vivo
Reactivity:
Human
Species/Host:
Human
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg, 1 mg

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

P19438

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols