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Human VCAM-1
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Tag | C-6His |
| Molecular Weight | Predicted: 75.26 KDa. Observed: 85-120 KDa, reducing conditions |
| Expression Region | Phe25-Glu698 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM CaCl2, 2mM MgCl2, 5% Threhalose, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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1.56-100ng/mL
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48 T, 96 THuman VCAM-1 [orb2992986]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 101.2 kDa. Observed: 115-145 kDa, reducing conditions
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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 101.4 KDa. Observed: 100-130 KDa, reducing conditions
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Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 76.9 KDa. Observed: 90-125 KDa, reducing conditions
20 μg, 100 μg

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Protocol Information
Human VCAM-1 (orb2995156)
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