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Human VEGF 121
SKU: orb2994925
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Human VEGF 121(Cat#orb2994925) at 2μg/ml (100 μl/well) can bind Human VEGFR2-Fc(Cat#orb2994455).The ED50 of Human VEGFR2-Fc(Cat#orb2994455) is 13.04 ng/ml.. BLI: Loaded Human VEGF Antibody (5E7)-Fc(Cat#DA082) on AHC Biosensor, can bind Human VEGF 121(Cat#orb2994925) with an affinity constant of 1.31 nM as determined in BLI assay. |
| Tag | Tag free |
| Molecular Weight | Predicted: 14.2 KDa. Observed: 16 KDa, reducing conditions |
| Expression Region | Ala27-Arg147 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Vascular endothelial growth factor A; VEGFA; VEGF-A; Vascular permeability factor; VPF; VEGF; VEGF121
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Human VEGF 121 (orb2994925)
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