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Recombinant Human /Cynomolgus CD28 Protein
SKU: orb2993961
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human/Cynomolgus |
| Biological Activity | ELISA: Immobilized Anti-CD28 mAb(Cat#A063) at 2μg/ml (100 μl/well) can bind Human/Cynomolgus CD28-Fc *(Cat#orb2993961)*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human/Cynomolgus CD28-Fc *(Cat#orb2993961) is 13.97 ng/ml. |
| Tag | C-Fc |
| Molecular Weight | Predicted: 42.3 KDa. Observed: 40-85 KDa, reducing conditions |
| Expression Region | Asn19-Pro152 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44
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Recombinant Human /Cynomolgus CD28 Protein (orb2993961)
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