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Mouse B7-1
SKU: orb2994276
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | BLI: Loaded Mouse CTLA-4-Fc(Cat#orb2994213) on Protein A Biosensor, can bind Mouse B7-1-His(Cat#orb2994276) with an affinity constant of 0.17 uM as determined in BLI assay. (Regularly tested) Loaded Mouse CD28-Fc-His(Cat#orb2994528) on Protein A Biosensor, can bind Mouse B7-1-His(Cat#orb2994276) with an affinity constant of 1.8 uM as determined in BLI assay. |
| Tag | C-6His |
| Molecular Weight | Predicted: 24.6 KDa. Observed: 38-55 KDa, reducing conditions |
| Expression Region | Val38-Asn246 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80
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Mouse B7-1 (orb2994276)
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