Recombinant Mouse B7-1 Protein

SKU: orb2994276

Description

Recombinant Mouse T-lymphocyte Activation Antigen CD80 is produced by our Mammalian expression system and the target gene encoding Val38-Asn246 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Mouse
Biological Activity	BLI: Loaded Mouse CTLA-4-Fc(Cat#orb2994213) on Protein A Biosensor, can bind Mouse B7-1-His(Cat#orb2994276) with an affinity constant of 0.17 uM as determined in BLI assay. (Regularly tested) Loaded Mouse CD28-Fc-His(Cat#orb2994528) on Protein A Biosensor, can bind Mouse B7-1-His(Cat#orb2994276) with an affinity constant of 1.8 uM as determined in BLI assay.
Tag	C-6His
Molecular Weight	Predicted: 24.6 KDa. Observed: 38-55 KDa, reducing conditions
Expression Region	Val38-Asn246
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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T-lymphocyte activation antigen CD80; Activation B7-1 antigen; B7; CD80

Human B7-1 Protein, mFc-His Tag [orb757420]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
Source:	E.coli

Sizes Available: 100 μg, 10 μg, 50 μg

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Mouse B7-1 Protein, hFc Tag [orb1290897]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 50 μg, 100 μg, 10 μg

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Recombinant mouse CD80 protein, N-GST & C-His [orb1516765]
Purity:
>90% as determined by SDS-PAGE
MW:
50.1 kDa
Sizes Available: 20 μg, 100 μg, 500 μg

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UniProt

Q00609

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No UniProt data available

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