Recombinant Mouse BAFF Protein

SKU: orb2993780

Description

Recombinant Mouse TNF Ligand Superfamily Member 13B is produced by our Mammalian expression system and the target gene encoding Ala127-Leu309 is expressed with a mFc tag at the N-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Mouse
Biological Activity	BLI: Loaded Human BCMA-His(Cat#orb2993882) on HIS1K Biosensor, can bind Mouse BAFF-mFc(Cat#orb2993780) with an affinity constant of 1.58 nM as determined in BLI assay. Loaded Cynomolgus BCMA-His(Cat#orb2993878) on HIS1K Biosensor, can bind Mouse BAFF-mFc(Cat#orb2993780) with an affinity constant of 0.32 nM as determined in BLI assay. Loaded Human BCMA-His(Cat#orb2994719) on HIS1K Biosensor, can bind Mouse BAFF-mFc(Cat#orb2993780) with an affinity constant of -3 nM as determined in BLI assay.
Tag	N-mFc
Molecular Weight	Predicted: 46.9 KDa. Observed: 45-60 KDa, reducing conditions
Expression Region	Ala127-Leu309
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Unconjugated

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of 20 mM Tris-HCl, 10% Trehalose, 2% Mannitol, 0.05% Tween 80, pH 8.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1

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Q9WU72

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